Accessory for NanoWizard AFMs
The Bruker JPK CellHesion Module is an add-on for NanoWizard AFMs, for measuring cell adhesion and cell mechanics.
- Long range force experiments with 100µm additional sample z-travel
- Measure single molecules, entire cells, tissue, organelles and embryos
- Simultaneous focus tracking (optional)
- Integrates with advanced optical imaging eg DIC, phase contrast, fluorescence, confocal microscopy, super-resolution, FRET, FLIM, etc.
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A single living cell is chemically bound to the cantilever sensor (eg with a fibronectin coating) under optical control.
The cell is brought into contact with the binding target on the substrate using a defined force. The binding target could be a molecular layer, implant surface, single cell or confluent monolayer. The substrate can be a slide, coverslip or Petri dish.
After a specified length of time, the cantilever is retracted vertically (on the z-axis) using a piezo actuator. This separates the cell from the substrate cell.
If there is adhesion, the cell resists the effort to remove it. This bends the cantilever, which is measured by a detector.
In physical terms, the cantilever is a leaf spring, so the adhesive force and energy can be calculated by measuring the bending.
This enables you to identify single-molecule binding events that contribute to the adhesion. By repeating the test with various cells, targets and conditions you can collect statistically relevant information.
The force vs. distance curve, enables you to calculate:
- Single molecule events
- “Work of removal”
- Tether formation
- Maximum adhesion force
- Viscoelastic parameters
- Stiffness and elasticity mapping
- Single cells to substrates and tissues
- Cell-cell and cell-substrate interaction
- Cell adhesion and tether formation
- Biomaterial studies, biofouling, biosensors, capsules
- Implant coatings and cellular biochips
- Microbiology and virus research
- Pharmaceuticals eg drug delivery mechanisms
- Food, paper and textiles eg fibres, coatings and powders in air or liquid
- Binding studies eg receptor/ligand and antibody/antigene
- Testing functionalised surfaces