New Ramp Scripting Package for Bruker BioScope Resolve with RampScript™

Stephen Badger AFM

New Ramp Scripting Package for Bruker BioScope Resolve AFM

Design extended time biological-dynamics experiments easily with RampScript, a new ramp scripting package for the Bruker BioScope Resolve bio-AFM. Study the dynamics of individual biomolecular bonds and the viscoelasticity of live cells with the widest range of ramp frequencies available.

Blue Scientific is the official Nordic distributor for Bruker Atomic Force Microscopes (Sweden, Denmark, Finland & Norway). If you have any questions or if you’d like a quote, please contact us:

 Contact us on +44 (0)1223 422 269 or info@blue-scientific.com

  

RampScript

We are excited about the advances in cell mechanobiology that will be enabled by the new ramp scripting capabilities for BioScope Resolve. Atomic force microscopy can play an even larger role in cell mechanics research when a system has the capability to correlate with the most accurate data to fluorescence. This is why we made it such a priority to add the most comprehensive biomechanics functionality to a system specifically designed for highest resolution on an inverted optical microscope.
Marco Tortonese, Ph.D., Vice President & General Manager of Bruker AFM

RampScript

RampScript is a powerful mechanical investigation tool set, which complements the BioScope Resolve’s wide range of ramp rates with extensive new scripting functionality. Build, control and record complex nanomechanical measurements in experiments such as protein pulling, ligand-receptor interaction and cell mechanics.

Features

  • User-definable scripts for custom point measurements
  • Step-by-step ramp definitions
  • Simple drag-and-drop functionality for the easy experiment control
  • Seamless switching from segment to segment between open- and closed-loop operation, ramping and holding, Z-feedback and force feedback
  • Add TTL signals easily to synchronise optical microscopy and other external measurements

RampScript also enables dynamic mechanical analysis. Perform frequency sweeps during ramp and hold measurements, both at single points as well as force volume map integration.

A specially-designed low-force trigger capability and fast, latency-free implementation deliver the most accurate script execution ever, with pN force control.

AFM on live cells

AFM of living MDCK cells. Left: PeakForce QNM topography. Right:Corresponding modulus image. Cell structures corresponding to actin fibers show higher modulus (lighter) while cell surface features, believed to be microvilli, appear softer (darker) than the cell membrane. 1 kHz PeakForce QNM, 32 µm image.

BioScope Resolve

The Bruker BioScope Resolve has set new standards in high resolution AFM imaging. It is the first instrument capable of imaging microvilli on live cells and routinely resolving sub-molecular structures, such as the major and minor groove of DNA on an inverted microscope.

The BioScope Resolve features the widest range of ramp rates for biomechanics, as well as Bruker’s Bruker’s exclusive PeakForce Tapping and FASTForce Volume data acquisition technologies. The new, flexible ramp scripting interface now makes new studies possible, so you can examine the dynamics of individual biomolecular bonds and the viscoelasticity of live cells.

Blue Scientific is the official Scandinavian distributor for Bruker Atomic Force Microscopes (Sweden, Denmark, Finland & Norway). If you would like more information please contact us:

 Contact us on +44 (0)1223 422 269 or info@blue-scientific.com